Elucidating the cellular mechanisms underlying adoptive cell transfer in models of glioma

Abstract Despite advances in solid tumor biology and oncology, pediatric adult primary brain cancer metastasis to the harbor dismal prognosis poor outcomes. One avenue overcome these clinical challenges is through developing robust immunotherapy better target tumor. Our group has previously shown that adoptive cellular therapy (ACT) provides therapeutic benefit against central nervous system (CNS) malignancies, namely medulloblastoma, stem glioma glioblastoma. In this study, we characterized cell cycling differences of immune sub-populations peripheral lymphoid tissue compartments vivo. ACT was conducted orthotopic KR158 luciferase tumor-bearing mice, a syngeneic murine model. Following completion ACT, bromodeoxyuridine (BRDU) administered mice 12-hours prior collection flow cytometry utilized measure level BRDU incorporation. 7-aminoactinomycin D used conjunction with provide insight into all phases cycle. We observed significant populations myeloid derived suppressor cells (MDSC) T cells. receiving MDSCs found secondary tissues were significantly more proliferative. microenvironment (TME), proliferating reduced compared did not receive treatment. Frequencies CD4+ CD8+ T-cells increased cervical lymph node TME, but spleen. These findings suggest changes migration dynamics TME potentially promote antitumor T-cell responses minimize tumor-mediated immunosuppression. 5R01NS112315-04